Part:BBa_K3478891:Design

p15A-Lac UV5-atoB-HMGS-HMGR-Trc-MK-PMK-PMD-idi

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 7338
  Illegal SpeI site found at 8475
  Illegal PstI site found at 2374
  Illegal PstI site found at 2602
  Illegal PstI site found at 5043
  Illegal PstI site found at 5334
  Illegal PstI site found at 5679
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 8475
  Illegal PstI site found at 2374
  Illegal PstI site found at 2602
  Illegal PstI site found at 5043
  Illegal PstI site found at 5334
  Illegal PstI site found at 5679
  Illegal NotI site found at 6171
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 7338
  Illegal SpeI site found at 8475
  Illegal PstI site found at 2374
  Illegal PstI site found at 2602
  Illegal PstI site found at 5043
  Illegal PstI site found at 5334
  Illegal PstI site found at 5679
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 7338
  Illegal SpeI site found at 8475
  Illegal PstI site found at 2374
  Illegal PstI site found at 2602
  Illegal PstI site found at 5043
  Illegal PstI site found at 5334
  Illegal PstI site found at 5679
  Illegal NgoMIV site found at 181
  Illegal NgoMIV site found at 3211
  Illegal NgoMIV site found at 3403
  Illegal NgoMIV site found at 3570
  Illegal NgoMIV site found at 4101
  Illegal NgoMIV site found at 4277
  Illegal NgoMIV site found at 6209
  Illegal AgeI site found at 3096
  Illegal AgeI site found at 6662
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5168
  Illegal BsaI site found at 7286
  Illegal BsaI.rc site found at 235
  Illegal BsaI.rc site found at 5106
  Illegal SapI.rc site found at 282
  Illegal SapI.rc site found at 3657
  Illegal SapI.rc site found at 4610
  Illegal SapI.rc site found at 5711
  

Design Notes

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Source

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